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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-30086 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAFG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse muscle, rat muscle.
- Concentration
- 0.53 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAFG using 30 µg of HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MAFG polyclonal antibody (Product # PA5-30086) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAFG was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAFG Polyclonal Antibody (Product # PA5-30086) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MAFG Polyclonal Antibody (Product # PA5-30086). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG Polyclonal Antibody (Product # PA5-30086) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFG Polyclonal Antibody detects MAFG protein by western blot analysis. A.50 µg mouse muscle lysate/extract.12% SDS-PAGE. MAFG Polyclonal Antibody (Product # PA5-30086) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFG Polyclonal Antibody detects MAFG protein by western blot analysis. A.50 µg rat muscle lysate/extract.12% SDS-PAGE. MAFG Polyclonal Antibody (Product # PA5-30086) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MAFG was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: MAFG Polyclonal Antibody (Product # PA5-30086) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using MAFG (Product # PA5-30086) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MAFG was performed in HeLa whole cell extracts using 5 µg of MAFG Polyclonal Antibody (Product # PA5-30086). Samples were transferred to a membrane and probed with MAFG Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Mouse IgG was used as a secondary antibody.
