AP2745d

antibody from Abgent

Targeting: LGR5 FEX, GPR49, GPR67, HG38

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2745d

Provider

Abgent

Proper citation

Abgent Cat#AP2745d, RRID:AB_2281170

Product name

LGR5 Antibody (loop2)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

LGR5 is a promising biomarker for patients with stage I and II gastric cancer.

Bu Z, Zheng Z, Zhang L, Li Z, Sun Y, Dong B, Wu A, Wu X, Wang X, Cheng X, Xing X, Li Y, Du H, Ji J
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2013 Feb;25(1):79-89

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Intestinal stem cell marker LGR5 expression during gastric carcinogenesis.

Zheng ZX, Sun Y, Bu ZD, Zhang LH, Li ZY, Wu AW, Wu XJ, Wang XH, Cheng XJ, Xing XF, Du H, Ji JF
World journal of gastroenterology 2013 Dec 14;19(46):8714-21

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Transcription factor Oct1 is a somatic and cancer stem cell determinant.

Maddox J, Shakya A, South S, Shelton D, Andersen JN, Chidester S, Kang J, Gligorich KM, Jones DA, Spangrude GJ, Welm BE, Tantin D
PLoS genetics 2012;8(11):e1003048

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Significance of Lgr5(+ve) cancer stem cells in the colon and rectum.

Takahashi H, Ishii H, Nishida N, Takemasa I, Mizushima T, Ikeda M, Yokobori T, Mimori K, Yamamoto H, Sekimoto M, Doki Y, Mori M
Annals of surgical oncology 2011 Apr;18(4):1166-74

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Stable expression of neurogenin 1 induces LGR5, a novel stem cell marker, in an immortalized human neural stem cell line HB1.F3.

Satoh J, Obayashi S, Tabunoki H, Wakana T, Kim SU
Cellular and molecular neurobiology 2010 Apr;30(3):415-26

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

LGR5/GPR49 Antibody (loop2) (Cat.#AP2745d) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-LGR5/GPR49 Antibody (loop2) (Cat.#AP2745d) in mouse cerebellum tissue lysates (35ug/lane).LGR5/GPR49(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of HeLa cells stained with LGR5 (loop2) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2745d LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000139292)."

Primary Ab dilution

1:100

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (loop2) (Cat.#AP2745d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (loop2) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50