MAB8078

antibody from R&D Systems

Targeting: LGR5 FEX, GPR49, GPR67, HG38

Antibody data

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Validation data

Product number: MAB8078 - Provider product page
Provider: R&D Systems
Product name: Human Lgr5/GPR49 Antibody
Antibody type: Monoclonal
Description: Protein A or G purified from hybridoma culture supernatant. Detects human Lgr5/GPR49 in ELISA. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: O75473
Isotype: IgG
Antibody clone number: 707042
Vial size: 100 ug
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

LGR5 protein structure - MAB8078 shown in red.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Lgr5/GPR49 in NSO Mouse Cell Line Transfected with Human Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed NSO mouse myeloma cell line transfected with GFP (green) tagged human LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Lgr5/GPR49 in Mouse Cells Transfected with Mouse Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm. Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Lgr5/GPR49 in Human Cells Transfected with Human Lgr5/GPR49. Lgr5/GPR49 was detected in MYC tagged human transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm. Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.