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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2591 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2591, RRID:AB_1575728
- Product name
- LGR5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of LGR5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of stem cells in small intestine and colon by marker gene Lgr5.
Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors.
Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations.
Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H
Nature 2007 Oct 25;449(7165):1003-7
Nature 2007 Oct 25;449(7165):1003-7
Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors.
McClanahan T, Koseoglu S, Smith K, Grein J, Gustafson E, Black S, Kirschmeier P, Samatar AA
Cancer biology & therapy 2006 Apr;5(4):419-26
Cancer biology & therapy 2006 Apr;5(4):419-26
Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations.
Yamamoto Y, Sakamoto M, Fujii G, Tsuiji H, Kenetaka K, Asaka M, Hirohashi S
Hepatology (Baltimore, Md.) 2003 Mar;37(3):528-33
Hepatology (Baltimore, Md.) 2003 Mar;37(3):528-33
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of LGR5 polyclonal antibody (Cat # PAB2591) in K-562 cell line lysates (35 ug/lane). LGR5 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5 polyclonal antibody (Cat # PAB2591), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
