- TA503316 - OriGene LGR5 antibody

Submitted references Full-length LGR5-positive cells have chemoresistant characteristics in colorectal cancer.

Osawa H, Takahashi H, Nishimura J, Ohta K, Haraguchi N, Hata T, Yamamoto H, Mizushima T, Takemasa I, Doki Y, Mori M
British journal of cancer 2016 May 24;114(11):1251-60

LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal.

Shaikh LH, Zhou J, Teo AE, Garg S, Neogi SG, Figg N, Yeo GS, Yu H, Maguire JJ, Zhao W, Bennett MR, Azizan EA, Davenport AP, McKenzie G, Brown MJ
The Journal of clinical endocrinology and metabolism 2015 Jun;100(6):E836-44

CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression.

Hsu HC, Liu YS, Tseng KC, Yang TS, Yeh CY, You JF, Hung HY, Chen SJ, Chen HC
Journal of cancer research and clinical oncology 2015 Jan;141(1):11-21

LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/β-catenin signaling pathway in colorectal cancer cells.

Hsu HC, Liu YS, Tseng KC, Tan BC, Chen SJ, Chen HC
Cellular signalling 2014 Nov;26(11):2333-42

Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro.

Wang J, Guo X, Lui M, Chu PJ, Yoo J, Chang M, Yen Y
PloS one 2014 Jan 17;9(1):e85112

Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis.

Jiang N, Zhou J, Chen M, Schiff MD, Lee CH, Kong K, Embree MC, Zhou Y, Mao JJ
Biomaterials 2014 Feb;35(7):2172-80

Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers.

Wu C, Xie Y, Gao F, Wang Y, Guo Y, Tian H, Li Y, Fan W
Gene 2013 Aug 1;525(1):18-25

PKH26 staining defines distinct subsets of normal human colon epithelial cells at different maturation stages.

Pastò A, Marchesi M, Diamantini A, Frasson C, Curtarello M, Lago C, Pilotto G, Parenti AR, Esposito G, Agostini M, Nitti D, Amadori A
PloS one 2012;7(8):e43379

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Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Anti-LGR5 mouse monoclonal antibody (TA503316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5(RC212825).
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (TA503316, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Validation comment: IP

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40?magnified (A) and 400? magnified (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of the glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Figure from citation: Positive correlation between LSD1, c-Myc, ?-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, ?-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: HEK293T cells transfected with either RC212825 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody(TA503316), and then analyzed by flow cytometry.
Validation comment: FC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Figure from citation: Cytofluorimetric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values (6 SD) of 5 consecutive experiments. Dilution: 1:400
Validation comment: FC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Figure from citation: Flow cytometry of the SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of the P2 population. Black: staining with Lgr5; red: staining with the isotype control.
Validation comment: FC

TA503316

Antibody data