AP12376PU-N

antibody from Acris Antibodies GmbH

Targeting: LGR5 FEX, GPR49, GPR67, HG38

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP12376PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP12376PU-N, RRID:AB_1769179

Product name

anti LGR5 / GPR49 (Loop2)

Antibody type

Polyclonal

Antigen

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689-719 amino acids from the loop2 region of Human LGR5/GPR49.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.4 ml

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of anti-LGR5/GPR49 Antibody (loop2) (Cat.#AP12376PU-N) in mouse cerebellum tissue lysates (35ug/lane).LGR5/GPR49(arrow) was detected using the purified Pab.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of anti-LGR5/GPR49 Antibody (loop2) (Cat.#AP12376PU-N) in in HepG2 cell line lysates (35ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of anti-LGR5/GPR49 Antibody (loop2) (Cat.#AP12376PU-N) in in HepG2 cell line lysates (35ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).(Kindly offered by Dr. Li).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of anti-LGR5/GPR49 Antibody (loop2) (Cat.#AP12376PU-N) in Hela and HCEC cell line lysates. This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow) (Kindly offered by Dr. Xiaolin Zhou from UNMC-Eppley Institute).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Fluorescent confocal image of HeLa cells stained with LGR5 (loop2) antibody AP12376PU-N. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2745d LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

(LEFT) Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (loop2) (Cat.#AP12376PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for Immunohistochemistry. Clinical relevance has not been evaluated. (RIGHT) Immunofluorescence analysis of anti-LGR5/GPR49 Antibody (loop2) (Cat#AP12376PU-N) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated Donkey anti-Rabbit lgG (H+L). Alexa-Fluor-546 emits orange Fluorescence.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow Cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (loop2) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.