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LearnH00008837-M01
antibody from Invitrogen Antibodies
Targeting: CFLAR
c-FLIP, CASH, CASP8AP1, Casper, cFLIP, CLARP, FLAME, FLIP, I-FLICE, MRIT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00008837-M01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CFLAR Monoclonal Antibody (2F12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: AGTSYRNVLQ AAIQKSLKDP SNNFRLHNGR SKEQRLKEQL GAQQEPVKKS IQESEAFLPQ SIPEERYKMK SKPLGICLII DCIGNETELL RDTFTSLGYE
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2F12
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CFLAR expression in transfected 293T cell line by CFLAR monoclonal antibody (M01), clone 2F12.Lane 1: CFLAR transfected lysate (Predicted MW: 55.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CFLAR monoclonal antibody (M01), clone 2F12. Western Blot analysis of CFLAR expression in K-562.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CFLAR expression in transfected 293T cell line by CFLAR monoclonal antibody (M01), clone 2F12.Lane 1: CFLAR transfected lysate (Predicted MW: 55.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CFLAR monoclonal antibody (M01), clone 2F12 Western Blot analysis of CFLAR expression in IMR-32 (Cat # L008V1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CFLAR is approximately 0.3 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CFLAR on HeLa cell. Antibody concentration is 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CFLAR on formalin-fixed paraffin-embedded human kidney. Antibody concentration is 3 µg/mL.
