PA5-21858

antibody from Invitrogen Antibodies

Targeting: TRAK2 ALS2CR3, CALS-C, GRIF-1, KIAA0549, MILT2, OIP98

Provider product page for PA5-21858

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Western blot [1]
Other assay [3]

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Product number: PA5-21858 - Provider product page
Provider: Invitrogen Antibodies
Product name: TRAK2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
Concentration: 1 mg/mL

TRAK2 protein structure - PA5-21858 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Effects of caspase-cleaved tau on kinesin 1 and dynein expression in immortalized cortical neurons. Panels (A,B) show representative western blot images of CN 1.4 cells transfected with GFP, GFP-T4 (normal tau), and GFP-T4C3 (truncated tau) showing the relative kinesin 1 (KIF5) or dynein expression levels. Also, Panels (A',B') show a quantitative analysis of the relative expression levels of kinesin 1 (A') and dynein (B') in immortalized cells transfected with GFP and GFP-tau (s) forms. Data are mean +- SE, n = 3. Total RNA was extracted from immortalized cortical neurons transfected with GFP, GFP-T4 (full-length tau), and GFP-T4C3 (truncated tau), and mRNA expression levels of kinesin (A'') and dynein (B'') were measured by RT-PCR (Jara et al., ). Data represent mean +- SE, n = 4.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Truncated tau reduces TRAK2 expression and increases its localization in the neuronal soma of hippocampal neurons. (A) Representative immunofluorescence images of rat hippocampal neurons transfected with GFP, GFP-T4 (normal tau), and GFP-T4C3 (truncated tau) showing TRAK2 expression and mitochondrial staining (MitoTracker Red CMXRos, a fixable mitochondrial dye). Expression of GFP-T4C3 reduces TRAK2 levels compared with neurons expressing GFP-T4 (normal tau). (B) Higher magnification images from (A) showing a detail of the TRAK2 localization and expression pattern in hippocampal neurons transfected with the indicated conditions. Cells transfected with caspase-cleaved tau show an apparent reduction in TRAK2 expression and an apparent increase in the localization of TRAK2 with MitoTracker Red CMXRos, suggesting an increase in the association of TRAK2 with mitochondria. White arrows indicate presence of mitochondria in neuronal processes, which is reduced in neurons that expressed caspase-cleaved tau. (C) Quantitative analysis of TRAK2 fluorescence levels obtained from hippocampal neurons transfected with GFP, GFP-T4, and GFP-T4C3. Truncated tau reduced TRAK2 expression compared to neurons that expressed full-length tau (GFP-T4). Data are mean +- SE, n = 4. * p < 0.05 indicates differences between groups calculated by ANOVA test. Immunofluorescence studies are representative of four independent experiments. Bar = 20 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Truncated tau reduces TRAK2 expression and increases its association with mitochondrial fraction in immortalized cortical neurons. (A) Representative western blot images of CN 1.4 cells transfected with GFP, GFP-T4 (normal tau), and GFP-T4C3 (truncated tau) indicating the relative TRAK2 expression levels. Caspase-cleaved tau decreases TRAK2 expression compared with cells that were expressing GFP or GFP-T4 (normal tau). (A') Quantitative analysis of the relative expression levels of TRAK2 in immortalized neurons transfected with indicated conditions. Data are mean +- SE, n = 5. * p < 0.05 indicates differences between groups calculated by ANOVA test. (A'') Total RNA was extracted from immortalized cortical neurons transfected with GFP, GFP-T4 (full-length tau), and GFP-T4C3 (truncated tau) and mRNA expression levels of TRAK2 were measured by RT-PCR. Data represent mean +- SE, n = 3. (B) Representative western blot images of CN 1.4 cells for mitochondrial extracts transfected with the conditions indicated. (B') Quantitative graph showing that caspase-cleaved tau expression increases TRAK2 presence in mitochondrial extracts compared with cells transfected with the full-length tau form (GFP-T4). Data are mean +- SE, n = 4. * p < 0.05 indicates differences between groups calculated by ANOVA test. (C) Representative western blot images and quantitative analysis (C') of kinesin 1 protein levels in mitochondrial extracts of CN 1.4 cells transfected with the conditions indica