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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MAB4832 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD28 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CD28 in ELISA.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P31041- Isotype
- IgG
- Antibody clone number
- 794716
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Crucial role of OX40/OX40L signaling in a murine model of asthma.
Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression.
The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells.
Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCRβ Repertoire.
Lei W, Zeng D, Liu G, Zhu Y, Wang J, Wu H, Jiang J, Huang J
Molecular medicine reports 2018 Mar;17(3):4213-4220
Molecular medicine reports 2018 Mar;17(3):4213-4220
Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression.
Fu X, Sun F, Wang F, Zhang J, Zheng B, Zhong J, Yue T, Zheng X, Xu JF, Wang CY
Mediators of inflammation 2017;2017:5706152
Mediators of inflammation 2017;2017:5706152
The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells.
Pan W, Hao WT, Shen YJ, Li XY, Wang YJ, Sun FF, Yin JH, Zhang J, Tang RX, Cao JP, Zheng KY
Parasites & vectors 2017 Jul 21;10(1):348
Parasites & vectors 2017 Jul 21;10(1):348
Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCRβ Repertoire.
Li KP, Fähnrich A, Roy E, Cuda CM, Grimes HL, Perlman HR, Kalies K, Hildeman DA
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):257-269
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):257-269
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD28 in CD3+ Mouse Splenocytes by Flow Cytometry. CD3+ mouse splenocytes were stained with Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4832, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mouse CD28 Antibody Induces Proliferation in Mouse T Cells. Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4832) induces proliferation in mouse T cells in the presence of 100 ng/mL Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484), in a dose dependent manner, as measured by Resazurin (Catalog # AR002), The ED50 for this effect is typically 0.07-0.42 μg/mL.
