GTX113412

antibody from GeneTex

Targeting: DBT BCKAD-E2, BCKDH-E2, BCOADC-E2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX113412

Provider

GeneTex

Proper citation

GeneTex Cat#GTX113412, RRID:AB_2036741

Product name

DBT antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

DBT antibody detects DBT protein by western blot analysis.A. 50 ?g rat liver lysate/extract 10% SDS-PAGEDBT antibody (GTX113412) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: H1299 B: HepG2 (GTX27900) 10% SDS PAGE GTX113412 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

DBT antibody detects DBT protein by western blot analysis.A. 50 ?g mouse liver lysate/extract 10% SDS-PAGEDBT antibody (GTX113412) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using DBT(GTX113412) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

DBT antibody detects DBT protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. DBT antibody (GTX113412) diluted at 1:500.