NBP1-31760

antibody from Novus Biologicals

Targeting: DBT BCKAD-E2, BCKDH-E2, BCOADC-E2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-31760

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31760, RRID:AB_2230317

Product name

Rabbit Polyclonal DBT Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DBT Antibody [NBP1-31760] - Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug mouse liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DBT Antibody [NBP1-31760] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DBT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-31760] - Sample: Paraffin-embedded mouse kidney. DBT antibody diluted at 1:500.