Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PAB7221 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7221, RRID:AB_1578343
- Product name
- P4HA1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of P4HA1.
- Antigen sequence
C-DFARKDEPDAFKE
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Hypoxia potentiates microRNA-mediated gene silencing through posttranslational modification of Argonaute2.
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
Wu C, So J, Davis-Dusenbery BN, Qi HH, Bloch DB, Shi Y, Lagna G, Hata A
Molecular and cellular biology 2011 Dec;31(23):4760-74
Molecular and cellular biology 2011 Dec;31(23):4760-74
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI
The Journal of biological chemistry 1997 Jul 11;272(28):17342-8
The Journal of biological chemistry 1997 Jul 11;272(28):17342-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- P4HA1 polyclonal antibody (Cat # PAB7221) (0.2 ug/mL) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.