14755-1-AP

antibody from Invitrogen Antibodies

Targeting: ACAT2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14755-1-AP

Provider

Invitrogen Antibodies

Product name

ACAT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNAGSDPVV IVSAARTIIG SFNGALAAVP VQDLGSTVIK EVLKRATVAP EDVSEVIFGH VLAAGCGQNP VRQASVGAGI PYSVPAWSCQ MICGSGLKAV CLAVQSIGIG DSSIVVAGGM ENMSKAPHLA YLRTGVKIGE MPLTDSILCD GLTDAFHNCH MGITAENVAK KWQVSREDQD KVAVLSQNRT ENAQKAGHFD KEIVPVLVST RRGLIEVKTD EFPRHGSNIE AMSKLKPYFL TDGTGTVTPA NASGINDGAA AVVLMKKSEA DKRGLTPLAR IVSWSQVGVE PSIMGIGPIP AIKQAVTKAG WSLEDVDIFE INEAFAAVSA AIVKELGLNP EKVNIEGGAI ALGHPLGASG CRILVTLLHT LERMGRSRGV AALCIGGGMG IAMCVQRE (1-397 aa encoded by BC000408)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 14755-1-AP ( ACAT2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 14755-1-AP (ACAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug ACAT2 antibody (14755-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ACAT2 (IP:14755-1-AP, 4ug; Detection:14755-1-AP 1:1000) with mouse liver tissue lysate 4000ug.