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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-25040 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JHDM1D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Glioblastoma initiating cells are sensitive to histone demethylase inhibition due to epigenetic deregulation.
Statin and Bisphosphonate Induce Starvation in Fast-Growing Cancer Cell Lines.
Mallm JP, Windisch P, Biran A, Gal Z, Schumacher S, Glass R, Herold-Mende C, Meshorer E, Barbus M, Rippe K
International journal of cancer 2020 Mar 1;146(5):1281-1292
International journal of cancer 2020 Mar 1;146(5):1281-1292
Statin and Bisphosphonate Induce Starvation in Fast-Growing Cancer Cell Lines.
Karlic H, Haider F, Thaler R, Spitzer S, Klaushofer K, Varga F
International journal of molecular sciences 2017 Sep 15;18(9)
International journal of molecular sciences 2017 Sep 15;18(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a JHDM1D polyclonal antibody (Product # PA5-25040) in 293 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human brain tissue using a JHDM1D polyclonal antibody (Product # PA5-25040), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of 293 cells using a JHDM1D polyclonal antibody (Product # PA5-25040) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 The effect of a simvastatin and ibandronate (treatment for 6 days with indicated EC50 concentrations as shown in Table 5 ) on the protein expression of the histone demethylase KDM7A is shown. The photographs show KDM7A (95 kD) in the upper lanes and the actin ACTA1 (42 kD) in the lower lanes for comparisons. ""Con"" refers to untreated control cells, ""Ibn"" to ibandronate-treated cells, and ""Sim"" to simvastatin-treated cells. Chemo-luminescence for the analysis of protein bands was measured with an image acquisition. Results of chemiluminescence measurements are given as means of three immunoblots.
