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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10304 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10304, RRID:AB_1676254
- Product name
- PACRG polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PACRG.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Susceptibility to leprosy is associated with PARK2 and PACRG.
Identification of a novel gene linked to parkin via a bi-directional promoter.
A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death.
Mira MT, Alcaïs A, Nguyen VT, Moraes MO, Di Flumeri C, Vu HT, Mai CP, Nguyen TH, Nguyen NB, Pham XK, Sarno EN, Alter A, Montpetit A, Moraes ME, Moraes JR, Doré C, Gallant CJ, Lepage P, Verner A, Van De Vosse E, Hudson TJ, Abel L, Schurr E
Nature 2004 Feb 12;427(6975):636-40
Nature 2004 Feb 12;427(6975):636-40
Identification of a novel gene linked to parkin via a bi-directional promoter.
West AB, Lockhart PJ, O'Farell C, Farrer MJ
Journal of molecular biology 2003 Feb 7;326(1):11-9
Journal of molecular biology 2003 Feb 7;326(1):11-9
A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death.
Imai Y, Soda M, Murakami T, Shoji M, Abe K, Takahashi R
The Journal of biological chemistry 2003 Dec 19;278(51):51901-10
The Journal of biological chemistry 2003 Dec 19;278(51):51901-10
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using PACRG polyclonal antibody (Cat # PAB10304) shows detection of aband ~ 40 KDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this bandis blocked when the antibody is pre-incubated withthe immunizing peptide (Lane 2).Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed byLI-COR.IRDye is a trademark of LI-COR, Inc.
