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Learn600-401-474
antibody from Rockland Immunochemicals, Inc.
Targeting: PACRG
FLJ32724, Glup, HAK005771, PARK2CRG
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 600-401-474 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-474, RRID:AB_2298959
- Product name
- Anti-PACRG (RABBIT) Antibody - 600-401-474
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Proteasomal inhibition reduces parkin mRNA in PC12 and SH-SY5Y cells.
Koch A, Lehmann-Horn K, Dächsel JC, Gasser T, Kahle PJ, Lücking CB
Parkinsonism & related disorders 2009 Mar;15(3):220-5
Parkinsonism & related disorders 2009 Mar;15(3):220-5
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Affinity Purified anti-PACRG antibody shows detection of a band ~40 kDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot
