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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- GTX48698 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48698, RRID:AB_11168710
- Product name
- ABCB6 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Canine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1,400. Personal Communication, Jill Paterson, CCR-NCI, Bethesda, MD.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag? antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1,400.
