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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- PAB9926 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9926, RRID:AB_1672067
- Product name
- ABCB6 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ABCB6.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MTABC3, a novel mitochondrial ATP-binding cassette protein involved in iron homeostasis.
Mitsuhashi N, Miki T, Senbongi H, Yokoi N, Yano H, Miyazaki M, Nakajima N, Iwanaga T, Yokoyama Y, Shibata T, Seino S
The Journal of biological chemistry 2000 Jun 9;275(23):17536-40
The Journal of biological chemistry 2000 Jun 9;275(23):17536-40
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using ABCB6 polyclonal antibody (Cat # PAB9926) (right panel, lane B6) shows detectionof Flag™-tagged human ABCB6 proteinat 104 KDa and a truncated form of theprotein at 79 KDa (arrowheads).The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein.A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody.The left panel shows a similar staining pattern using an anti-Flag™ antibody asa positive control.The membrane was probed with the primary antibody dilutedto 1 : 1,400.Personal Communication, Jill Paterson, CCR-NCI, Bethesda, MD.
