600-401-945

antibody from Rockland Immunochemicals, Inc.

Targeting: ABCB6 EST45597, MTABC3, umat

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-945

Provider

Rockland Immunochemicals, Inc.

Product name

Anti-ABCB6 (RABBIT) Antibody - 600-401-945

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy.

Nakayama T, Otsuka S, Kobayashi T, Okajima H, Matsumoto K, Hagiya Y, Inoue K, Shuin T, Nakajima M, Tanaka T, Ogura SI
Scientific reports 2016 Nov 18;6:36478

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag™ antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1,400. Personal Communication, Jill Paterson, CCR-NCI, Bethesda, MD.

Validation comment

Western Blot