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LearnNB300-229
antibody from Novus Biologicals
Targeting: SYCP1
CT8, HOM-TES-14, SCP1
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [43]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB300-229 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-229, RRID:AB_10002742
- Product name
- Rabbit Polyclonal SCP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cytogenetic mechanisms of unisexuality in rock lizards.
Germ cell-intrinsic effects of sex chromosomes on early oocyte differentiation in mice.
NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.
Mouse DCUN1D1 (SCCRO) is required for spermatogenetic individualization.
The pericentromeric heterochromatin of homologous chromosomes remains associated after centromere pairing dissolves in mouse spermatocyte meiosis.
FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis.
Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis.
CXXC1 is not essential for normal DNA double-strand break formation and meiotic recombination in mouse.
Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination.
SHOC1 is a ERCC4-(HhH)2-like protein, integral to the formation of crossover recombination intermediates during mammalian meiosis.
A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL).
Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice.
Rnf138 deficiency promotes apoptosis of spermatogonia in juvenile male mice.
Efficient TALEN-mediated gene targeting of chicken primordial germ cells.
Sororin is enriched at the central region of synapsed meiotic chromosomes.
Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I.
Reduced MAD2 levels dampen the apoptotic response to non-exchange sex chromosomes and lead to sperm aneuploidy.
Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis.
PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis.
Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice.
Fasciola hepatica: a light and electron microscope study of the ovary and of the development of oocytes within eggs in the uterus provides an insight into reproductive strategy.
Sex chromosome recombination failure, apoptosis, and fertility in male mice.
ALDH Enzyme Expression Is Independent of the Spermatogenic Cycle, and Their Inhibition Causes Misregulation of Murine Spermatogenic Processes.
Re-engineering the zinc fingers of PRDM9 reverses hybrid sterility in mice.
Nuclear localization of PRDM9 and its role in meiotic chromatin modifications and homologous synapsis.
The histone demethylase KDM1A is essential for the maintenance and differentiation of spermatogonial stem cells and progenitors.
An ENU-induced mutation in the mouse Rnf212 gene is associated with male meiotic failure and infertility.
Lack of sex chromosome specific meiotic silencing in platypus reveals origin of MSCI in therian mammals.
Identification and characterisation of synaptonemal complex genes in monotremes.
The dual role of HOP2 in mammalian meiotic homologous recombination.
In vitro spermatogenesis using an organ culture technique.
Mouse HFM1/Mer3 is required for crossover formation and complete synapsis of homologous chromosomes during meiosis.
Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice.
HORMAD2 is essential for synapsis surveillance during meiotic prophase via the recruitment of ATR activity.
HORMAD1-dependent checkpoint/surveillance mechanism eliminates asynaptic oocytes.
Targeted inactivation of nuclear interaction partner of ALK disrupts meiotic prophase.
Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.
In vitro production of fertile sperm from murine spermatogonial stem cell lines.
Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.
Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes.
Regulation of the meiotic prophase I to metaphase I transition in mouse spermatocytes.
Heritable and stable gene knockdown in rats.
Spangenberg V, Arakelyan M, Cioffi MB, Liehr T, Al-Rikabi A, Martynova E, Danielyan F, Stepanyan I, Galoyan E, Kolomiets O
Scientific reports 2020 May 26;10(1):8697
Scientific reports 2020 May 26;10(1):8697
Germ cell-intrinsic effects of sex chromosomes on early oocyte differentiation in mice.
Hamada N, Hamazaki N, Shimamoto S, Hikabe O, Nagamatsu G, Takada Y, Kato K, Hayashi K
PLoS genetics 2020 Mar;16(3):e1008676
PLoS genetics 2020 Mar;16(3):e1008676
NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.
Zhang B, Tang Z, Li L, Lu LY
Cell death and differentiation 2020 Jul;27(7):2176-2190
Cell death and differentiation 2020 Jul;27(7):2176-2190
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.
Zhang Y, Schroeder LK, Lessard MD, Kidd P, Chung J, Song Y, Benedetti L, Li Y, Ries J, Grimm JB, Lavis LD, De Camilli P, Rothman JE, Baddeley D, Bewersdorf J
Nature methods 2020 Feb;17(2):225-231
Nature methods 2020 Feb;17(2):225-231
Mouse DCUN1D1 (SCCRO) is required for spermatogenetic individualization.
Huang G, Kaufman AJ, Ryan RJH, Romin Y, Huryn L, Bains S, Manova-Todorova K, Morris PL, Hunnicutt GR, Adelman CA, Petrini JHJ, Ramanathan Y, Singh B
PloS one 2019;14(1):e0209995
PloS one 2019;14(1):e0209995
The pericentromeric heterochromatin of homologous chromosomes remains associated after centromere pairing dissolves in mouse spermatocyte meiosis.
Eyster C, Chuong HH, Lee CY, Pezza RJ, Dawson D
Chromosoma 2019 Sep;128(3):355-367
Chromosoma 2019 Sep;128(3):355-367
FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis.
Hua R, Wei H, Liu C, Zhang Y, Liu S, Guo Y, Cui Y, Zhang X, Guo X, Li W, Liu M
Nucleic acids research 2019 Dec 16;47(22):11755-11770
Nucleic acids research 2019 Dec 16;47(22):11755-11770
Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis.
Lam KG, Brick K, Cheng G, Pratto F, Camerini-Otero RD
Nature communications 2019 Aug 23;10(1):3821
Nature communications 2019 Aug 23;10(1):3821
CXXC1 is not essential for normal DNA double-strand break formation and meiotic recombination in mouse.
Tian H, Billings T, Petkov PM
PLoS genetics 2018 Oct;14(10):e1007657
PLoS genetics 2018 Oct;14(10):e1007657
Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination.
Abreu CM, Prakash R, Romanienko PJ, Roig I, Keeney S, Jasin M
Nature communications 2018 Oct 10;9(1):3961
Nature communications 2018 Oct 10;9(1):3961
SHOC1 is a ERCC4-(HhH)2-like protein, integral to the formation of crossover recombination intermediates during mammalian meiosis.
Guiraldelli MF, Felberg A, Almeida LP, Parikh A, de Castro RO, Pezza RJ
PLoS genetics 2018 May;14(5):e1007381
PLoS genetics 2018 May;14(5):e1007381
A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL).
Tsang M, Gantchev J, Netchiporouk E, Moreau L, Ghazawi FM, Glassman S, Sasseville D, Litvinov IV
Oncotarget 2018 Dec 28;9(102):37647-37661
Oncotarget 2018 Dec 28;9(102):37647-37661
Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice.
Miyauchi H, Ohta H, Nagaoka S, Nakaki F, Sasaki K, Hayashi K, Yabuta Y, Nakamura T, Yamamoto T, Saitou M
The EMBO journal 2017 Nov 2;36(21):3100-3119
The EMBO journal 2017 Nov 2;36(21):3100-3119
Rnf138 deficiency promotes apoptosis of spermatogonia in juvenile male mice.
Xu L, Lu Y, Han D, Yao R, Wang H, Zhong S, Luo Y, Han R, Li K, Fu J, Zong S, Miao S, Song W, Wang L
Cell death & disease 2017 May 18;8(5):e2795
Cell death & disease 2017 May 18;8(5):e2795
Efficient TALEN-mediated gene targeting of chicken primordial germ cells.
Taylor L, Carlson DF, Nandi S, Sherman A, Fahrenkrug SC, McGrew MJ
Development (Cambridge, England) 2017 Mar 1;144(5):928-934
Development (Cambridge, England) 2017 Mar 1;144(5):928-934
Sororin is enriched at the central region of synapsed meiotic chromosomes.
Jordan PW, Eyster C, Chen J, Pezza RJ, Rankin S
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2017 Jun;25(2):115-128
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2017 Jun;25(2):115-128
Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I.
Casey AE, Daish TJ, Barbero JL, Grützner F
Scientific reports 2017 Jun 26;7(1):4217
Scientific reports 2017 Jun 26;7(1):4217
Reduced MAD2 levels dampen the apoptotic response to non-exchange sex chromosomes and lead to sperm aneuploidy.
Faisal I, Kauppi L
Development (Cambridge, England) 2017 Jun 1;144(11):1988-1996
Development (Cambridge, England) 2017 Jun 1;144(11):1988-1996
Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis.
Dai J, Voloshin O, Potapova S, Camerini-Otero RD
Cell reports 2017 Feb 7;18(6):1383-1394
Cell reports 2017 Feb 7;18(6):1383-1394
PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis.
Parvanov ED, Tian H, Billings T, Saxl RL, Spruce C, Aithal R, Krejci L, Paigen K, Petkov PM
Molecular biology of the cell 2017 Feb 1;28(3):488-499
Molecular biology of the cell 2017 Feb 1;28(3):488-499
Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice.
Fu C, Begum K, Jordan PW, He Y, Overbeek PA
PloS one 2016;11(8):e0159800
PloS one 2016;11(8):e0159800
Fasciola hepatica: a light and electron microscope study of the ovary and of the development of oocytes within eggs in the uterus provides an insight into reproductive strategy.
Hanna RE, Moffett D, Forster FI, Trudgett AG, Brennan GP, Fairweather I
Veterinary parasitology 2016 May 15;221:93-103
Veterinary parasitology 2016 May 15;221:93-103
Sex chromosome recombination failure, apoptosis, and fertility in male mice.
Faisal I, Kauppi L
Chromosoma 2016 Jun;125(2):227-35
Chromosoma 2016 Jun;125(2):227-35
ALDH Enzyme Expression Is Independent of the Spermatogenic Cycle, and Their Inhibition Causes Misregulation of Murine Spermatogenic Processes.
Kent T, Arnold SL, Fasnacht R, Rowsey R, Mitchell D, Hogarth CA, Isoherranen N, Griswold MD
Biology of reproduction 2016 Jan;94(1):12
Biology of reproduction 2016 Jan;94(1):12
Re-engineering the zinc fingers of PRDM9 reverses hybrid sterility in mice.
Davies B, Hatton E, Altemose N, Hussin JG, Pratto F, Zhang G, Hinch AG, Moralli D, Biggs D, Diaz R, Preece C, Li R, Bitoun E, Brick K, Green CM, Camerini-Otero RD, Myers SR, Donnelly P
Nature 2016 Feb 11;530(7589):171-176
Nature 2016 Feb 11;530(7589):171-176
Nuclear localization of PRDM9 and its role in meiotic chromatin modifications and homologous synapsis.
Sun F, Fujiwara Y, Reinholdt LG, Hu J, Saxl RL, Baker CL, Petkov PM, Paigen K, Handel MA
Chromosoma 2015 Sep;124(3):397-415
Chromosoma 2015 Sep;124(3):397-415
The histone demethylase KDM1A is essential for the maintenance and differentiation of spermatogonial stem cells and progenitors.
Lambrot R, Lafleur C, Kimmins S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Nov;29(11):4402-16
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Nov;29(11):4402-16
An ENU-induced mutation in the mouse Rnf212 gene is associated with male meiotic failure and infertility.
Fujiwara Y, Matsumoto H, Akiyama K, Srivastava A, Chikushi M, Ann Handel M, Kunieda T
Reproduction (Cambridge, England) 2015 Jan;149(1):67-74
Reproduction (Cambridge, England) 2015 Jan;149(1):67-74
Lack of sex chromosome specific meiotic silencing in platypus reveals origin of MSCI in therian mammals.
Daish TJ, Casey AE, Grutzner F
BMC biology 2015 Dec 10;13:106
BMC biology 2015 Dec 10;13:106
Identification and characterisation of synaptonemal complex genes in monotremes.
Casey AE, Daish TJ, Grutzner F
Gene 2015 Aug 10;567(2):146-53
Gene 2015 Aug 10;567(2):146-53
The dual role of HOP2 in mammalian meiotic homologous recombination.
Pezza RJ, Voloshin ON, Volodin AA, Boateng KA, Bellani MA, Mazin AV, Camerini-Otero RD
Nucleic acids research 2014 Feb;42(4):2346-57
Nucleic acids research 2014 Feb;42(4):2346-57
In vitro spermatogenesis using an organ culture technique.
Yokonishi T, Sato T, Katagiri K, Ogawa T
Methods in molecular biology (Clifton, N.J.) 2013;927:479-88
Methods in molecular biology (Clifton, N.J.) 2013;927:479-88
Mouse HFM1/Mer3 is required for crossover formation and complete synapsis of homologous chromosomes during meiosis.
Guiraldelli MF, Eyster C, Wilkerson JL, Dresser ME, Pezza RJ
PLoS genetics 2013 Mar;9(3):e1003383
PLoS genetics 2013 Mar;9(3):e1003383
Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice.
Sato T, Yokonishi T, Komeya M, Katagiri K, Kubota Y, Matoba S, Ogonuki N, Ogura A, Yoshida S, Ogawa T
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 16;109(42):16934-8
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 16;109(42):16934-8
HORMAD2 is essential for synapsis surveillance during meiotic prophase via the recruitment of ATR activity.
Kogo H, Tsutsumi M, Inagaki H, Ohye T, Kiyonari H, Kurahashi H
Genes to cells : devoted to molecular & cellular mechanisms 2012 Nov;17(11):897-912
Genes to cells : devoted to molecular & cellular mechanisms 2012 Nov;17(11):897-912
HORMAD1-dependent checkpoint/surveillance mechanism eliminates asynaptic oocytes.
Kogo H, Tsutsumi M, Ohye T, Inagaki H, Abe T, Kurahashi H
Genes to cells : devoted to molecular & cellular mechanisms 2012 Jun;17(6):439-54
Genes to cells : devoted to molecular & cellular mechanisms 2012 Jun;17(6):439-54
Targeted inactivation of nuclear interaction partner of ALK disrupts meiotic prophase.
Illert AL, Kawaguchi H, Antinozzi C, Bassermann F, Quintanilla-Martinez L, von Klitzing C, Hiwatari M, Peschel C, de Rooij DG, Morris SW, Barchi M, Duyster J
Development (Cambridge, England) 2012 Jul;139(14):2523-34
Development (Cambridge, England) 2012 Jul;139(14):2523-34
Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.
La Salle S, Palmer K, O'Brien M, Schimenti JC, Eppig J, Handel MA
Biology of reproduction 2012 Feb;86(2):45
Biology of reproduction 2012 Feb;86(2):45
In vitro production of fertile sperm from murine spermatogonial stem cell lines.
Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T
Nature communications 2011 Sep 13;2:472
Nature communications 2011 Sep 13;2:472
Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.
Sun F, Palmer K, Handel MA
Development (Cambridge, England) 2010 May;137(10):1699-707
Development (Cambridge, England) 2010 May;137(10):1699-707
Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes.
Yoshimura T, Toyoda S, Kuramochi-Miyagawa S, Miyazaki T, Miyazaki S, Tashiro F, Yamato E, Nakano T, Miyazaki J
Developmental biology 2009 Nov 1;335(1):216-27
Developmental biology 2009 Nov 1;335(1):216-27
Regulation of the meiotic prophase I to metaphase I transition in mouse spermatocytes.
Sun F, Handel MA
Chromosoma 2008 Oct;117(5):471-85
Chromosoma 2008 Oct;117(5):471-85
Heritable and stable gene knockdown in rats.
Dann CT, Alvarado AL, Hammer RE, Garbers DL
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 25;103(30):11246-51
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 25;103(30):11246-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.
