500-P95A-100UG
antibody from Invitrogen Antibodies
Targeting: CCL20
CKb4, exodus-1, LARC, MIP-3a, SCYA20, ST38
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- 500-P95A-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIP-3 alpha Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM.
- Concentration
- 0.1-1.0 mg/mL
Submitted references Involvement of CCR6/CCL20/IL-17 axis in NSCLC disease progression.
Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
Kirshberg S, Izhar U, Amir G, Demma J, Vernea F, Beider K, Shlomai Z, Wald H, Zamir G, Shapira OM, Peled A, Wald O
PloS one 2011;6(9):e24856
PloS one 2011;6(9):e24856
Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
Kriehuber E, Breiteneder-Geleff S, Groeger M, Soleiman A, Schoppmann SF, Stingl G, Kerjaschki D, Maurer D
The Journal of experimental medicine 2001 Sep 17;194(6):797-808
The Journal of experimental medicine 2001 Sep 17;194(6):797-808
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect HumanMIP-3alpha by Western Blot analysis MIP-3 alpha Polyclonal Antibody (Product # 500-P95A-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect HumanMIP-3alpha by Western Blot analysis MIP-3 alpha Polyclonal Antibody (Product # 500-P95A-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human MIP-3alpha (CCL20) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of MIP-3 alpha Polyclonal Antibody (Product # 500-P95A-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech MIP-3 alpha Polyclonal Antibody, Biotin (Product # 500-P95ABT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3alpha (CCL20).