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LearnGTX15669
antibody from GeneTex
Targeting: CD58
LFA3
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX15669 - Provider product page
- Provider
- GeneTex
- Product name
- CD58 antibody [TS2/9]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of CD58 in 25 ug of Raji, Jurkat and BAF-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4¢XC overnight. The membrane was probed with CD58/LFA-3 antibody [TS2/9] at a dilution of 1:100 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD58 (green) in Raji cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CD58/LFA-3 antibody [TS2/9] in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4¢XC in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
