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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM31149PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM31149PU-N, RRID:AB_10974131
- Product name
- anti CD58 / LFA3
- Antibody type
- Monoclonal
- Antigen
- CD58 (AAH05930, 1 a.a. ~ 241 a.a) full length recombinant protein with GST tag.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D11-B10
- Vial size
- 50 µg
- Concentration
- 0.37 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of CD58 expression in Jurkat using CD58 Antibody Cat.-No AM31149PU-N (Clone 2D11-B10).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CD58 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal CD58 Antibody Cat.-No AM31149PU-N (Clone 2D11-B10) on HeLa cell at 10 µg/ml.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded Human Thymus stained with CD58 Antibody Cat.-No AM31149PU-N (Clone 2D11-B10) at 5 µg/ml after heat-induced antigen retrieval
