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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- H00006672-B01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006672-B01, RRID:AB_982933
- Product name
- SP100 MaxPab mouse polyclonal antibody (B01)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human SP100 protein.
- Antigen sequence
MAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQ
RMFTEDQGVDDRLLYDIVFKHFKRNKVEISNAIKK
TFPFLEGLRDRDLITNKMFEDSQDSCRNLVPVQRV
VYNVLSELEKTFNLPVLEALFSDVNMQEYPDLIHI
YKGFENVIHDKLPLQESEEEEREERSGLQLSLEQG
TGENSFRSLTWPPSGSPSHAGTTPPENGLSEHPCE
TEQINAKRKDTTSDKDDSLGSQQTNEQCAQKAEPT
ESCEQIAVQVNNGDAGREMPCPLPCDEESPEAELH
NHGIQINSCSVRLVDIKKEKPFSNSKVECQAQART
HHNQASDIIVISSEDSEGSTDVDEPLEVFISAPRS
EPVINNDNPLESNDEKEGQEATCSRPQIVPEPMDF
RKLSTFRESFKKRVIGQDHDFSESSEEEAPAEASS
GALRSKHGEKAPMTSRSTSTWRIPSRKRRFSSSDF
SDLSNGEELQETCSSSLRRGSGKED- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Contribution of the Major ND10 Proteins PML, hDaxx and Sp100 to the Regulation of Human Cytomegalovirus Latency and Lytic Replication in the Monocytic Cell Line THP-1.
Kaposi's sarcoma associated herpesvirus tegument protein ORF75 is essential for viral lytic replication and plays a critical role in the antagonization of ND10-instituted intrinsic immunity.
Wagenknecht N, Reuter N, Scherer M, Reichel A, Müller R, Stamminger T
Viruses 2015 Jun 5;7(6):2884-907
Viruses 2015 Jun 5;7(6):2884-907
Kaposi's sarcoma associated herpesvirus tegument protein ORF75 is essential for viral lytic replication and plays a critical role in the antagonization of ND10-instituted intrinsic immunity.
Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, Stürzl M, Myoung J, Jung JU, Stamminger T, Ensser A
PLoS pathogens 2014 Jan;10(1):e1003863
PLoS pathogens 2014 Jan;10(1):e1003863
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SP100 expression in transfected 293T cell line (H00006672-T01) by SP100 MaxPab polyclonal antibody.Lane 1: SP100 transfected lysate(52.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of purified MaxPab antibody to SP100 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
