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Learn6672-MSM1-P0
antibody from Invitrogen Antibodies
Targeting: SP100
Western blot ELISA
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 6672-MSM1-P0 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP100 Monoclonal Antibody (PCRP-SP100-1B9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PCRP-SP100-1B9
- Vial size
- 20 µg
- Concentration
- 200 µg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PFA-fixed HeLa cells stained using SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IHC analysis of Formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using PCRP-SP100-1B9 at 2 µg/mL in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2 °: HRP-polymer, 30 min. DAB, 5 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IHC analysis of Formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using PCRP-SP100-1B9 at 2 µg/mL in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2 °: HRP-polymer, 30 min. DAB, 5 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PFA-fixed HeLa cells. SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Analysis of Protein Array containing more than 19,000 full-length human proteins using SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the diFerence (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDS-PAGE analysis Purified SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9). Confirmation of Purity and Integrity of Antibody.
