PA5-55689

antibody from Invitrogen Antibodies

Targeting: CHD1L ALC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55689

Provider

Invitrogen Antibodies

Product name

Anti-CHD1L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AWWESNNYQS FCLPSEESEP EDLENGEESS AELDYQDPDA TSLKYVSGDV THPQAGAEDA LIVHCVDDSG HWGRGGLFTA LEKRSAEPR Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 80%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CHD1L in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a CHD1L Polyclonal Antibody (Product # PA5-55689). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CHD1L using CHD1L Polyclonal Antibody (Product # PA5-55689) (A) shows similar pattern to an independent CHD1L Polyclonal Antibody (B).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of CHD1L in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a CHD1L Polyclonal Antibody (Product # PA5-55689).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CHD1L in human testis and pancreas tissues using CHD1L Polyclonal Antibody (Product # PA5-55689). Corresponding CHD1L RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CHD1L in human cerebral cortex, colon, pancreas and testis using CHD1L Polyclonal Antibody (Product # PA5-55689) (A) shows similar protein distribution across tissues to an independent CHD1L Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CHD1L in human pancreas using CHD1L Polyclonal Antibody (Product # PA5-55689) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CHD1L in human colon using CHD1L Polyclonal Antibody (Product # PA5-55689).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CHD1L in human cerebral cortex using CHD1L Polyclonal Antibody (Product # PA5-55689).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CHD1L in human testis tissue shows distinct nuclear positivity in germ cells of testis tissue. Samples were probed using a CHD1L Polyclonal Antibody (Product # PA5-55689).