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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- TA302225 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Antibody against TRPM8 (Center R536)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against TRPM8 (Center R536)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- TRPM8
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
Submitted references Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion.
Yee NS, Li Q, Kazi AA, Yang Z, Berg A, Yee RK
Cells 2014 May 23;3(2):500-16
Cells 2014 May 23;3(2):500-16
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of TRPM8 (arrow) using rabbit polyclonal TRPM8 Antibody (Center R536)(Cat.#TA302225).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2) (Origene Technologies).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from human brain tissue lysate, using TRPM8 Antibody (Center R536)(Cat. #TA302225). TA302225 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of TRPM8 protein level by using anti-TRPM8 antibody in human pancreatic adenocarcinoma cell lines. Dilution: 1:750
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody (Center R536) (Cat.#TA302225), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
