Explore
Validate
LearnNBP2-20321
antibody from Novus Biologicals
Targeting: SETDB1
ESET, KG1T, KIAA0067, KMT1E, TDRD21
Western blot Immunoprecipitation
Immunohistochemistry Blocking/Neutralizing Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-20321 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SETDB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Silica nanoparticles induce cytokine responses in lung epithelial cells through activation of a p38/TACE/TGF-α/EGFR-pathway and NF-κΒ signalling.
Skuland T, Ovrevik J, Låg M, Schwarze P, Refsnes M
Toxicology and applied pharmacology 2014 Aug 15;279(1):76-86
Toxicology and applied pharmacology 2014 Aug 15;279(1):76-86
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SETDB1 Antibody [NBP2-20321] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:2000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SETDB1 Antibody [NBP2-20321] - IP samples: HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N1], N-term 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent].
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: SETDB1 Antibody [NBP2-20321] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to P53P2.
