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- Antibody Data
- Antigen structure
- References [45]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 11231-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11231-1-AP, RRID:AB_2186069
- Product name
- SETDB1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SETDB1 antibody (Cat. #11231-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Expression of SET domain bifurcated histone lysine methyltransferase 1 and its clinical prognostic significance in hepatocellular carcinoma.
KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements.
KAP1-Mediated Epigenetic Suppression in Anti-RNA Viral Responses by Direct Targeting RIG-I and MDA5.
The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.
METTL3 regulates heterochromatin in mouse embryonic stem cells.
Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.
TRIM28 inhibits alternative lengthening of telomere phenotypes by protecting SETDB1 from degradation.
ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.
METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.
ATRX limits the accessibility of histone H3-occupied HSV genomes during lytic infection.
The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase.
Defect of SLC38A3 promotes epithelial-mesenchymal transition and predicts poor prognosis in esophageal squamous cell carcinoma.
PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat.
SETDB1 promotes the progression of colorectal cancer via epigenetically silencing p21 expression.
DICER regulates the expression of major satellite repeat transcripts and meiotic chromosome segregation during spermatogenesis.
Hdac3, Setdb1, and Kap1 mark H3K9me3/H3K14ac bivalent regions in young and aged liver.
SETDB1 Inhibits p53-Mediated Apoptosis and Is Required for Formation of Pancreatic Ductal Adenocarcinomas in Mice.
High SET Domain Bifurcated 1 (SETDB1) Expression Predicts Poor Prognosis in Breast Carcinoma.
AFF3-DNA methylation interplay in maintaining the mono-allelic expression pattern of XIST in terminally differentiated cells.
SETDB1 induces epithelial‑mesenchymal transition in breast carcinoma by directly binding with Snail promoter.
AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions.
ATF7IP regulates SETDB1 nuclear localization and increases its ubiquitination.
SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.
Melatonin protects mouse spermatogonial stem cells against hexavalent chromium-induced apoptosis and epigenetic histone modification.
Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.
SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice.
SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling.
Histone Methyltransferase SETDB1 Promotes the Progression of Colorectal Cancer by Inhibiting the Expression of TP53.
Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway.
Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.
A Histone Methyltransferase ESET Is Critical for T Cell Development.
ATF7IP-Mediated Stabilization of the Histone Methyltransferase SETDB1 Is Essential for Heterochromatin Formation by the HUSH Complex.
Activation of Endogenous Retroviruses in Dnmt1(-/-) ESCs Involves Disruption of SETDB1-Mediated Repression by NP95 Binding to Hemimethylated DNA.
Hypomethylation of ERVs in the sperm of mice haploinsufficient for the histone methyltransferase Setdb1 correlates with a paternal effect on phenotype.
SETDB1 accelerates tumourigenesis by regulating the WNT signalling pathway.
KMT1E-mediated chromatin modifications at the FcγRIIb promoter regulate thymocyte development.
GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
The histone methyltransferase SETDB1 represses endogenous and exogenous retroviruses in B lymphocytes.
Setdb1 is required for myogenic differentiation of C2C12 myoblast cells via maintenance of MyoD expression.
Reactivation of maternal SNORD116 cluster via SETDB1 knockdown in Prader-Willi syndrome iPSCs.
The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice.
Methionine adenosyltransferase II-dependent histone H3K9 methylation at the COX-2 gene locus.
Functional analysis of KAP1 genomic recruitment.
ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes.
SetDB1 contributes to repression of genes encoding developmental regulators and maintenance of ES cell state.
Wang C, Xia Z, Li Z, Ye F, Ji S, Lu C, Zhang H
Journal of clinical laboratory analysis 2022 Jan;36(1):e24090
Journal of clinical laboratory analysis 2022 Jan;36(1):e24090
KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements.
Zhang SM, Cai WL, Liu X, Thakral D, Luo J, Chan LH, McGeary MK, Song E, Blenman KRM, Micevic G, Jessel S, Zhang Y, Yin M, Booth CJ, Jilaveanu LB, Damsky W, Sznol M, Kluger HM, Iwasaki A, Bosenberg MW, Yan Q
Nature 2021 Oct;598(7882):682-687
Nature 2021 Oct;598(7882):682-687
KAP1-Mediated Epigenetic Suppression in Anti-RNA Viral Responses by Direct Targeting RIG-I and MDA5.
Li Q, Qin Y, Wang W, Jia M, Zhao W, Zhao C
Journal of immunology (Baltimore, Md. : 1950) 2021 Oct 1;207(7):1903-1910
Journal of immunology (Baltimore, Md. : 1950) 2021 Oct 1;207(7):1903-1910
The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.
Liu J, Gao M, He J, Wu K, Lin S, Jin L, Chen Y, Liu H, Shi J, Wang X, Chang L, Lin Y, Zhao YL, Zhang X, Zhang M, Luo GZ, Wu G, Pei D, Wang J, Bao X, Chen J
Nature 2021 Mar;591(7849):322-326
Nature 2021 Mar;591(7849):322-326
METTL3 regulates heterochromatin in mouse embryonic stem cells.
Xu W, Li J, He C, Wen J, Ma H, Rong B, Diao J, Wang L, Wang J, Wu F, Tan L, Shi YG, Shi Y, Shen H
Nature 2021 Mar;591(7849):317-321
Nature 2021 Mar;591(7849):317-321
Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.
Griffin GK, Wu J, Iracheta-Vellve A, Patti JC, Hsu J, Davis T, Dele-Oni D, Du PP, Halawi AG, Ishizuka JJ, Kim SY, Klaeger S, Knudsen NH, Miller BC, Nguyen TH, Olander KE, Papanastasiou M, Rachimi S, Robitschek EJ, Schneider EM, Yeary MD, Zimmer MD, Jaffe JD, Carr SA, Doench JG, Haining WN, Yates KB, Manguso RT, Bernstein BE
Nature 2021 Jul;595(7866):309-314
Nature 2021 Jul;595(7866):309-314
TRIM28 inhibits alternative lengthening of telomere phenotypes by protecting SETDB1 from degradation.
Wang C, Songyang Z, Huang Y
Cell & bioscience 2021 Jul 30;11(1):149
Cell & bioscience 2021 Jul 30;11(1):149
ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.
Ma L, Yu L, Jiang BC, Wang J, Guo X, Huang Y, Ren J, Sun N, Gao DS, Ding H, Lu J, Zhou H, Zou L, Gao Y, Wang L, Sun K, Ming Y, Meng Z, Tao YX, Yan M
The Journal of experimental medicine 2021 Dec 6;218(12)
The Journal of experimental medicine 2021 Dec 6;218(12)
METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.
Orellana EA, Liu Q, Yankova E, Pirouz M, De Braekeleer E, Zhang W, Lim J, Aspris D, Sendinc E, Garyfallos DA, Gu M, Ali R, Gutierrez A, Mikutis S, Bernardes GJL, Fischer ES, Bradley A, Vassiliou GS, Slack FJ, Tzelepis K, Gregory RI
Molecular cell 2021 Aug 19;81(16):3323-3338.e14
Molecular cell 2021 Aug 19;81(16):3323-3338.e14
ATRX limits the accessibility of histone H3-occupied HSV genomes during lytic infection.
Cabral JM, Cushman CH, Sodroski CN, Knipe DM
PLoS pathogens 2021 Apr;17(4):e1009567
PLoS pathogens 2021 Apr;17(4):e1009567
The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase.
Li X, Chen X, Liu Y, Zhang P, Zheng Y, Zeng W
Frontiers in genetics 2020;11:997
Frontiers in genetics 2020;11:997
Defect of SLC38A3 promotes epithelial-mesenchymal transition and predicts poor prognosis in esophageal squamous cell carcinoma.
Liu R, Hong R, Wang Y, Gong Y, Yeerken D, Yang D, Li J, Fan J, Chen J, Zhang W, Zhan Q
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2020 Oct 31;32(5):547-563
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2020 Oct 31;32(5):547-563
PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat.
He Z, Jing S, Yang T, Chen J, Huang F, Zhang W, Peng Z, Liu B, Ma X, Wu L, Pan T, Zhang X, Li L, Cai W, Tang X, Zhang J, Zhang H
Journal of virology 2020 May 4;94(10)
Journal of virology 2020 May 4;94(10)
SETDB1 promotes the progression of colorectal cancer via epigenetically silencing p21 expression.
Cao N, Yu Y, Zhu H, Chen M, Chen P, Zhuo M, Mao Y, Li L, Zhao Q, Wu M, Ye M
Cell death & disease 2020 May 11;11(5):351
Cell death & disease 2020 May 11;11(5):351
DICER regulates the expression of major satellite repeat transcripts and meiotic chromosome segregation during spermatogenesis.
Yadav RP, Mäkelä JA, Hyssälä H, Cisneros-Montalvo S, Kotaja N
Nucleic acids research 2020 Jul 27;48(13):7135-7153
Nucleic acids research 2020 Jul 27;48(13):7135-7153
Hdac3, Setdb1, and Kap1 mark H3K9me3/H3K14ac bivalent regions in young and aged liver.
Price AJ, Manjegowda MC, Kain J, Anandh S, Bochkis IM
Aging cell 2020 Feb;19(2):e13092
Aging cell 2020 Feb;19(2):e13092
SETDB1 Inhibits p53-Mediated Apoptosis and Is Required for Formation of Pancreatic Ductal Adenocarcinomas in Mice.
Ogawa S, Fukuda A, Matsumoto Y, Hanyu Y, Sono M, Fukunaga Y, Masuda T, Araki O, Nagao M, Yoshikawa T, Goto N, Hiramatsu Y, Tsuda M, Maruno T, Nakanishi Y, Hussein MS, Tsuruyama T, Takaori K, Uemoto S, Seno H
Gastroenterology 2020 Aug;159(2):682-696.e13
Gastroenterology 2020 Aug;159(2):682-696.e13
High SET Domain Bifurcated 1 (SETDB1) Expression Predicts Poor Prognosis in Breast Carcinoma.
Zhou Z, Wu B, Tang X, Yang W, Zou Q, Wang H
Medical science monitor : international medical journal of experimental and clinical research 2020 Apr 19;26:e922982
Medical science monitor : international medical journal of experimental and clinical research 2020 Apr 19;26:e922982
AFF3-DNA methylation interplay in maintaining the mono-allelic expression pattern of XIST in terminally differentiated cells.
Zhang Y, Wang C, Liu X, Yang Q, Ji H, Yang M, Xu M, Zhou Y, Xie W, Luo Z, Lin C
Journal of molecular cell biology 2019 Sep 19;11(9):761-769
Journal of molecular cell biology 2019 Sep 19;11(9):761-769
SETDB1 induces epithelial‑mesenchymal transition in breast carcinoma by directly binding with Snail promoter.
Yang W, Su Y, Hou C, Chen L, Zhou D, Ren K, Zhou Z, Zhang R, Liu X
Oncology reports 2019 Feb;41(2):1284-1292
Oncology reports 2019 Feb;41(2):1284-1292
AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions.
Guo J, Dai X, Laurent B, Zheng N, Gan W, Zhang J, Guo A, Yuan M, Liu P, Asara JM, Toker A, Shi Y, Pandolfi PP, Wei W
Nature cell biology 2019 Feb;21(2):226-237
Nature cell biology 2019 Feb;21(2):226-237
ATF7IP regulates SETDB1 nuclear localization and increases its ubiquitination.
Tsusaka T, Shimura C, Shinkai Y
EMBO reports 2019 Dec 5;20(12):e48297
EMBO reports 2019 Dec 5;20(12):e48297
SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.
Cossec JC, Theurillat I, Chica C, Búa Aguín S, Gaume X, Andrieux A, Iturbide A, Jouvion G, Li H, Bossis G, Seeler JS, Torres-Padilla ME, Dejean A
Cell stem cell 2018 Nov 1;23(5):742-757.e8
Cell stem cell 2018 Nov 1;23(5):742-757.e8
Melatonin protects mouse spermatogonial stem cells against hexavalent chromium-induced apoptosis and epigenetic histone modification.
Lv Y, Zhang P, Guo J, Zhu Z, Li X, Xu D, Zeng W
Toxicology and applied pharmacology 2018 Feb 1;340:30-38
Toxicology and applied pharmacology 2018 Feb 1;340:30-38
Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.
Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, Diehl WE, Luban J
Nature microbiology 2018 Dec;3(12):1354-1361
Nature microbiology 2018 Dec;3(12):1354-1361
SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice.
Hirota T, Blakeley P, Sangrithi MN, Mahadevaiah SK, Encheva V, Snijders AP, ElInati E, Ojarikre OA, de Rooij DG, Niakan KK, Turner JMA
Developmental cell 2018 Dec 3;47(5):645-659.e6
Developmental cell 2018 Dec 3;47(5):645-659.e6
SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling.
Mochizuki K, Tando Y, Sekinaka T, Otsuka K, Hayashi Y, Kobayashi H, Kamio A, Ito-Matsuoka Y, Takehara A, Kono T, Osumi N, Matsui Y
Development (Cambridge, England) 2018 Dec 3;145(23)
Development (Cambridge, England) 2018 Dec 3;145(23)
Histone Methyltransferase SETDB1 Promotes the Progression of Colorectal Cancer by Inhibiting the Expression of TP53.
Chen K, Zhang F, Ding J, Liang Y, Zhan Z, Zhan Y, Chen LH, Ding Y
Journal of Cancer 2017;8(16):3318-3330
Journal of Cancer 2017;8(16):3318-3330
Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway.
Liu T, Chen X, Li T, Li X, Lyu Y, Fan X, Zhang P, Zeng W
Biochimica et biophysica acta. Gene regulatory mechanisms 2017 Oct;1860(10):1094-1102
Biochimica et biophysica acta. Gene regulatory mechanisms 2017 Oct;1860(10):1094-1102
Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.
Tchasovnikarova IA, Timms RT, Douse CH, Roberts RC, Dougan G, Kingston RE, Modis Y, Lehner PJ
Nature genetics 2017 Jul;49(7):1035-1044
Nature genetics 2017 Jul;49(7):1035-1044
A Histone Methyltransferase ESET Is Critical for T Cell Development.
Takikita S, Muro R, Takai T, Otsubo T, Kawamura YI, Dohi T, Oda H, Kitajima M, Oshima K, Hattori M, Endo TA, Toyoda T, Weis J, Shinkai Y, Suzuki H
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2269-79
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2269-79
ATF7IP-Mediated Stabilization of the Histone Methyltransferase SETDB1 Is Essential for Heterochromatin Formation by the HUSH Complex.
Timms RT, Tchasovnikarova IA, Antrobus R, Dougan G, Lehner PJ
Cell reports 2016 Oct 11;17(3):653-659
Cell reports 2016 Oct 11;17(3):653-659
Activation of Endogenous Retroviruses in Dnmt1(-/-) ESCs Involves Disruption of SETDB1-Mediated Repression by NP95 Binding to Hemimethylated DNA.
Sharif J, Endo TA, Nakayama M, Karimi MM, Shimada M, Katsuyama K, Goyal P, Brind'Amour J, Sun MA, Sun Z, Ishikura T, Mizutani-Koseki Y, Ohara O, Shinkai Y, Nakanishi M, Xie H, Lorincz MC, Koseki H
Cell stem cell 2016 Jul 7;19(1):81-94
Cell stem cell 2016 Jul 7;19(1):81-94
Hypomethylation of ERVs in the sperm of mice haploinsufficient for the histone methyltransferase Setdb1 correlates with a paternal effect on phenotype.
Daxinger L, Oey H, Isbel L, Whitelaw NC, Youngson NA, Spurling A, Vonk KK, Whitelaw E
Scientific reports 2016 Apr 26;6:25004
Scientific reports 2016 Apr 26;6:25004
SETDB1 accelerates tumourigenesis by regulating the WNT signalling pathway.
Sun QY, Ding LW, Xiao JF, Chien W, Lim SL, Hattori N, Goodglick L, Chia D, Mah V, Alavi M, Kim SR, Doan NB, Said JW, Loh XY, Xu L, Liu LZ, Yang H, Hayano T, Shi S, Xie D, Lin DC, Koeffler HP
The Journal of pathology 2015 Mar;235(4):559-70
The Journal of pathology 2015 Mar;235(4):559-70
KMT1E-mediated chromatin modifications at the FcγRIIb promoter regulate thymocyte development.
Martin FJ, Xu Y, Lohmann F, Ciccone DN, Nicholson TB, Loureiro JJ, Chen T, Huang Q
Genes and immunity 2015 Mar;16(2):162-9
Genes and immunity 2015 Mar;16(2):162-9
GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
Tchasovnikarova IA, Timms RT, Matheson NJ, Wals K, Antrobus R, Göttgens B, Dougan G, Dawson MA, Lehner PJ
Science (New York, N.Y.) 2015 Jun 26;348(6242):1481-1485
Science (New York, N.Y.) 2015 Jun 26;348(6242):1481-1485
The histone methyltransferase SETDB1 represses endogenous and exogenous retroviruses in B lymphocytes.
Collins PL, Kyle KE, Egawa T, Shinkai Y, Oltz EM
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 7;112(27):8367-72
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 7;112(27):8367-72
Setdb1 is required for myogenic differentiation of C2C12 myoblast cells via maintenance of MyoD expression.
Song YJ, Choi JH, Lee H
Molecules and cells 2015 Apr;38(4):362-72
Molecules and cells 2015 Apr;38(4):362-72
Reactivation of maternal SNORD116 cluster via SETDB1 knockdown in Prader-Willi syndrome iPSCs.
Cruvinel E, Budinetz T, Germain N, Chamberlain S, Lalande M, Martins-Taylor K
Human molecular genetics 2014 Sep 1;23(17):4674-85
Human molecular genetics 2014 Sep 1;23(17):4674-85
The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice.
An J, Zhang X, Qin J, Wan Y, Hu Y, Liu T, Li J, Dong W, Du E, Pan C, Zeng W
Cell death & disease 2014 Apr 24;5:e1196
Cell death & disease 2014 Apr 24;5:e1196
Methionine adenosyltransferase II-dependent histone H3K9 methylation at the COX-2 gene locus.
Kera Y, Katoh Y, Ohta M, Matsumoto M, Takano-Yamamoto T, Igarashi K
The Journal of biological chemistry 2013 May 10;288(19):13592-601
The Journal of biological chemistry 2013 May 10;288(19):13592-601
Functional analysis of KAP1 genomic recruitment.
Iyengar S, Ivanov AV, Jin VX, Rauscher FJ 3rd, Farnham PJ
Molecular and cellular biology 2011 May;31(9):1833-47
Molecular and cellular biology 2011 May;31(9):1833-47
ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes.
Frietze S, O'Geen H, Blahnik KR, Jin VX, Farnham PJ
PloS one 2010 Dec 8;5(12):e15082
PloS one 2010 Dec 8;5(12):e15082
SetDB1 contributes to repression of genes encoding developmental regulators and maintenance of ES cell state.
Bilodeau S, Kagey MH, Frampton GM, Rahl PB, Young RA
Genes & development 2009 Nov 1;23(21):2484-9
Genes & development 2009 Nov 1;23(21):2484-9
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 11231-1-AP(SETDB1 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SETDB1 (IP:11231-1-AP, 3ug; Detection:11231-1-AP 1:1000) with MCF-7 cells lysate 2500ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 11231-1-AP(SETDB1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SETDB1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SETDB1. This antibody recognizes human, mouse antigen. The SETDB1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
