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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX115305 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115305, RRID:AB_11176036
- Product name
- SETDB1 antibody [N1], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Significance of histone methyltransferase SETDB1 expression in colon adenocarcinoma.
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Ho YJ, Lin YM, Huang YC, Chang J, Yeh KT, Lin LI, Gong Z, Tzeng TY, Lu JW
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2017 Nov;125(11):985-995
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2017 Nov;125(11):985-995
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Chen J, Liu H, Liu J, Qi J, Wei B, Yang J, Liang H, Chen Y, Chen J, Wu Y, Guo L, Zhu J, Zhao X, Peng T, Zhang Y, Chen S, Li X, Li D, Wang T, Pei D
Nature genetics 2013 Jan;45(1):34-42
Nature genetics 2013 Jan;45(1):34-42
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: U87-MG 5% SDS PAGE GTX115305 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SETDB1 antibody [N1], N-term immunoprecipitates SETDB1 protein in IP experiments.IP samples: HeLa nuclear extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of SETDB1 protein by 4 £gg SETDB1 antibody [N1], N-term (GTX115305)5 % SDS-PAGEThe immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to P53P2.
