PA5-120442

antibody from Invitrogen Antibodies

Targeting: BOK BCL2L9, BOKL, MGC4631

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120442

Provider

Invitrogen Antibodies

Product name

BOK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive test controls include: Mouse brain, Rat testis. The target is usually found in the following locations: Endoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass membrane protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

3.21 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of BOK in various cell lines using a BOK Polyclonal antibody (Product # PA5-120442) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of BOK in MCF7 cells using a BOK Polyclonal antibody (Product # PA5-120442). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of BOK in MCF7 cells using a BOK Polyclonal antibody (Product # PA5-120442). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BOK in mouse spleen using a Polyclonal antibody (Product # PA5-120442) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BOK in mouse spleen using a Polyclonal antibody (Product # PA5-120442) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BOK in mouse spleen using a Polyclonal antibody (Product # PA5-120442) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..