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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- TA806806 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA806806, RRID:AB_2628238
- Product name
- PD-1 (PDCD1) mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)
- Antibody type
- Monoclonal
- Description
- PD-1 (PDCD1) mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDCD1
- Isotype
- IgG
- Antibody clone number
- OTI4F10
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806806)(Left) . The rihgt is negative control.(1:100)(1:900)
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806806)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA806806),compared to a nonspecific negative control antibody (green),and then analyzed by f
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806806) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red),and detected by anti-Fc(human) IgG-FITC.(1:50)
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806806) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
- Validation comment
- FC
