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- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- AF1830 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-6R alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-6R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant mouse (rm) IL-6, recombinant human IL-6 R and rmgp130 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P22272- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references STAT-3-independent production of IL-17 by mouse innate-like αβ T cells controls ocular infection.
Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling.
Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130.
Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner.
Species specificity of ADAM10 and ADAM17 proteins in interleukin-6 (IL-6) trans-signaling and novel role of ADAM10 in inducible IL-6 receptor shedding.
Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice.
Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
Sequential ELISA to profile multiple cytokines from small volumes.
St Leger AJ, Hansen AM, Karauzum H, Horai R, Yu CR, Laurence A, Mayer-Barber KD, Silver P, Villasmil R, Egwuagu C, Datta SK, Caspi RR
The Journal of experimental medicine 2018 Apr 2;215(4):1079-1090
The Journal of experimental medicine 2018 Apr 2;215(4):1079-1090
Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling.
Arnold P, Boll I, Rothaug M, Schumacher N, Schmidt F, Wichert R, Schneppenheim J, Lokau J, Pickhinke U, Koudelka T, Tholey A, Rabe B, Scheller J, Lucius R, Garbers C, Rose-John S, Becker-Pauly C
Scientific reports 2017 Mar 9;7:44053
Scientific reports 2017 Mar 9;7:44053
Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130.
Wang X, Miller EB, Goswami M, Zhang P, Ronning KE, Karlen SJ, Zawadzki RJ, Pugh EN Jr, Burns ME
Journal of neuroinflammation 2017 Jun 23;14(1):121
Journal of neuroinflammation 2017 Jun 23;14(1):121
Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner.
Owusu-Boaitey N, Bauckman KA, Zhang T, Mysorekar IU
Immunity, inflammation and disease 2016 Dec;4(4):413-426
Immunity, inflammation and disease 2016 Dec;4(4):413-426
Species specificity of ADAM10 and ADAM17 proteins in interleukin-6 (IL-6) trans-signaling and novel role of ADAM10 in inducible IL-6 receptor shedding.
Garbers C, Jänner N, Chalaris A, Moss ML, Floss DM, Meyer D, Koch-Nolte F, Rose-John S, Scheller J
The Journal of biological chemistry 2011 Apr 29;286(17):14804-11
The Journal of biological chemistry 2011 Apr 29;286(17):14804-11
Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J
Blood 2008 Feb 1;111(3):1021-8
Blood 2008 Feb 1;111(3):1021-8
IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice.
Walker F, Zhang HH, Matthews V, Weinstock J, Nice EC, Ernst M, Rose-John S, Burgess AW
Blood 2008 Apr 15;111(8):3978-85
Blood 2008 Apr 15;111(8):3978-85
Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
Blood 2007 Sep 15;110(6):1748-55
Blood 2007 Sep 15;110(6):1748-55
Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
Kurooka M, Kaneda Y
Cancer research 2007 Jan 1;67(1):227-36
Cancer research 2007 Jan 1;67(1):227-36
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6R alpha in Mouse Thymus. IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6R alpha in Mouse Thymus. IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Mouse IL-6R alpha Antibody. Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND50 is typically 0.2-1 µg/mL.
