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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-31553 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PYGO2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: NIH-3T3.
- Concentration
- 0.65 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of pygopus 2 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a pygopus 2 polyclonal antibody (Product # PA5-31553) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PYGO2 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PYGO2 Polyclonal Antibody (Product # PA5-31553) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PYGO2 Polyclonal Antibody (Product # PA5-31553). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PYGO2 Polyclonal Antibody (Product # PA5-31553) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PYGO2 was performed in HCT 116 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PYGO2 Polyclonal Antibody (Product # PA5-31553) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PYGO2 was performed in paraffin-embedded mouse stomach tissue using PYGO2 Polyclonal Antibody (Product # PA5-31553) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PYGO2 was performed in paraffin-embedded rat intestine tissue using PYGO2 Polyclonal Antibody (Product # PA5-31553) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- (Product # PA5-31553) IP Image. pygopus 2 was immunoprecipitated from 5*107 cells of SW480 whole cell lysate using 1 µg of anti-pygopus 2 antibody (Product # PA5-31553). The precipitated pygopus 2 was detected by (Product # PA5-31553) diluted at 1:15000. A: IP-IgG . Lane B: IP-pygopus 2 antibody.
