M05260

antibody from Boster Biological Technology

Targeting: PYGO2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M05260

Provider

Boster Biological Technology

Product name

Anti-Pygopus 2 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for PYGOPUS 2/PYGO2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse PYGOPUS 2/PYGO2 information: Molecular Weight: 41244 MW; Subcellular Localization: Nucleus .

Reactivity

Human, Mouse

Host

Rabbit

Antibody clone number

ABI-16

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human uterus, using Pygopus 2 Antibody(M05260)PYGO2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PYGO2 Antibody (M05260)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image