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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-46171 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46171, RRID:AB_10009403
- Product name
- Rabbit Polyclonal Pygopus-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Mutations in Bcl9 and Pygo genes cause congenital heart defects by tissue-specific perturbation of Wnt/β-catenin signaling.
A cytoplasmic role of Wnt/β-catenin transcriptional cofactors Bcl9, Bcl9l, and Pygopus in tooth enamel formation.
The Pygo2-H3K4me2/3 interaction is dispensable for mouse development and Wnt signaling-dependent transcription.
Cantù C, Felker A, Zimmerli D, Prummel KD, Cabello EM, Chiavacci E, Méndez-Acevedo KM, Kirchgeorg L, Burger S, Ripoll J, Valenta T, Hausmann G, Vilain N, Aguet M, Burger A, Panáková D, Basler K, Mosimann C
Genes & development 2018 Nov 1;32(21-22):1443-1458
Genes & development 2018 Nov 1;32(21-22):1443-1458
A cytoplasmic role of Wnt/β-catenin transcriptional cofactors Bcl9, Bcl9l, and Pygopus in tooth enamel formation.
Cantù C, Pagella P, Shajiei TD, Zimmerli D, Valenta T, Hausmann G, Basler K, Mitsiadis TA
Science signaling 2017 Feb 7;10(465)
Science signaling 2017 Feb 7;10(465)
The Pygo2-H3K4me2/3 interaction is dispensable for mouse development and Wnt signaling-dependent transcription.
Cantù C, Valenta T, Hausmann G, Vilain N, Aguet M, Basler K
Development (Cambridge, England) 2013 Jun;140(11):2377-86
Development (Cambridge, England) 2013 Jun;140(11):2377-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pygopus-2 Antibody [NBP1-46171] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-Pygo2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Pygo2 was also immunoprecipitated by rabbit anti-Pygo2 antibody NBP1-46172, which recognizes a downstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP1-46171] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Pygo2 used at a dilution of 1:200 (1ug/ml) Detection: DAB
