PA5-30919

antibody from Invitrogen Antibodies

Targeting: RIT1 MGC125864, MGC125865, RIBB, RIT, ROC1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30919

Provider

Invitrogen Antibodies

Product name

RIT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431.

Concentration

0.75 mg/mL

References

Submitted references

Computational analysis of data from a genome-wide screening identifies new PARP1 functional interactors as potential therapeutic targets.

Lodovichi S, Mercatanti A, Cervelli T, Galli A
Oncotarget 2019 Apr 12;10(28):2722-2737

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RIT1 Polyclonal Antibody (Product # PA5-30919). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. RIT1 Polyclonal Antibody (Product # PA5-30919) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RIT1 Polyclonal Antibody detects RIT1 protein at cytosol on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT4838 xenograft. RIT1 Polyclonal Antibody (Product # PA5-30919) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Effect of RIT1, INCENP and PSAT1 on PARylation and PARP1 protein level, and olaparip sensitivity (A) Expression of INCENP, RIT1 and PSAT1 in triple negative and hormone receptor positive (+) breast cancer cell lines: (B) Expression of INCENP, RIT1 and PSAT1 in high grade serous and ovarian clear cell cancer lines. Data were compared and statically analyzed by Student's t -test as indicated: ns, not significant, * p