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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA800069 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800069, RRID:AB_2625195
- Product name
- CRABP2 mouse monoclonal antibody, clone OTI10D6 (formerly 10D6)
- Antibody type
- Monoclonal
- Description
- CRABP2 mouse monoclonal antibody, clone OTI10D6 (formerly 10D6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CRABP2
- Isotype
- IgG
- Antibody clone number
- OTI10D6
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Identification of Proteomic Features To Distinguish Benign Pulmonary Nodules from Lung Adenocarcinoma.
Codreanu SG, Hoeksema MD, Slebos RJC, Zimmerman LJ, Rahman SMJ, Li M, Chen SC, Chen H, Eisenberg R, Liebler DC, Massion PP
Journal of proteome research 2017 Sep 1;16(9):3266-3276
Journal of proteome research 2017 Sep 1;16(9):3266-3276
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRABP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRABP2 (RC200221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRABP2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRABP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800069)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200221 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CRABP2 antibody(TA800069), and then analyzed by flow cytometry.
- Validation comment
- FC
