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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 24639-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ISG20L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSTLLLNLD FGEPPPKKAL EGNAKHRNFV KKRRLLERRG FLSKKNQPPS KAPKLHSEPS KKGETPTVDG TWKTPSFPKK KTAASSNGSG QPLDKKAAVS WLTPAPSKKA DSVAAKVDLL GEFQSALPKI NSHPTRSQKK SSQKKSSKKN HPQKNAPQNS TQAHSENKCS GASQKLPRKM VAIDCEMVGT GPKGHVSSLA RCSIVNYNGD VLYDEYILPP CHIVDYRTRW SGIRKQHMVN ATPFKIARGQ ILKILTGKIV VGHAIHNDFK ALQYFHPKSL TRDTSHIPPL NRKADCPENA TMSLKHLTKK LLNRDIQVGK SGHSSVEDAQ ATMELYKLVE VEWEEHLARN PPTD (1-353 aa encoded by BC000575)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.47 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 24639-1-AP ( ISG20L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 24639-1-AP (ISG20L2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 24639-1-AP (ISG20L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ISG20L2 (IP:24639-1-AP, 4ug; Detection:24639-1-AP 1:1000) with HEK-293 cells lysate 3240ug.
