Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-15072 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Galactose protects against cell damage in mouse models of acute pancreatitis.
Peng S, Gerasimenko JV, Tsugorka TM, Gryshchenko O, Samarasinghe S, Petersen OH, Gerasimenko OV
The Journal of clinical investigation 2018 Aug 31;128(9):3769-3778
The Journal of clinical investigation 2018 Aug 31;128(9):3769-3778
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GCK using a GCK polyclonal antibody (Product # PA5-15072) in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of PANC-1 (Lane 1), Hep G2 (Lane 2), HeLa (Lane 3), tissue extracts Mouse Liver (Lane 4), Rat Liver (Lane 5), Rat Brain (Lane 6), Mouse Brain (Lane 7) and Mouse Kidney (Lane 8). The blot was probed with Anti-GCK Polyclonal Antibody (Product # PA5-15072, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 60 kDa band corresponding to GCK was observed across the cell lines and tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using a GCK polyclonal antibody (Product # PA5-15072) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using a GCK polyclonal antibody (Product # PA5-15072) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GCK was performed using 70% confluent log phase PANC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GCK Polyclonal Antibody (Product # PA5-15072) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a GCK polyclonal antibody (Product # PA5-15072), followed by HRP-conjugated secondary antibody and AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 HK activity is significantly inhibited in vitro by POA and BA. ( A ) HK1 activity is reduced significantly by 0.1 mM POA ( n = 6, P < 0.0001), but not changed significantly by 0.05% BA ( n = 4, P > 0.13) as compared with control ( n = 6). ( B ) HK2 activity is reduced significantly by 0.1 mM POA ( n = 9, P < 0.0001), but not affected by 0.05% BA ( n = 4, P > 0.3) as compared with control ( n = 13). ( C ) HK4 activity is reduced significantly by 0.05% BA ( n = 8, P < 0.0001), but not affected by 0.1 mM POA ( n = 4, P > 0.8) as compared with control ( n = 6). ( D ) Western blot analysis of the expression levels of HK1, HK2, and HK4 in PACs (representative case, repeated 3 times with similar results). *** P < 0.001, 1-way ANOVA.
