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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-32245 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-32245, RRID:AB_627890
- Product name
- Anti-STK11
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Regulation of ploidy and senescence by the AMPK-related kinase NUAK1.
SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase.
Dysfunctional AMPK activity, signalling through mTOR and survival in response to energetic stress in LKB1-deficient lung cancer
Humbert N, Navaratnam N, Augert A, Da Costa M, Martien S, Wang J, Martinez D, Abbadie C, Carling D, de Launoit Y, Gil J, Bernard D
The EMBO journal 2010 Jan 20;29(2):376-86
The EMBO journal 2010 Jan 20;29(2):376-86
SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase.
Hou X, Xu S, Maitland-Toolan KA, Sato K, Jiang B, Ido Y, Lan F, Walsh K, Wierzbicki M, Verbeuren TJ, Cohen RA, Zang M
The Journal of biological chemistry 2008 Jul 18;283(29):20015-26
The Journal of biological chemistry 2008 Jul 18;283(29):20015-26
Dysfunctional AMPK activity, signalling through mTOR and survival in response to energetic stress in LKB1-deficient lung cancer
J Carretero, P P Medina, R Blanco, L Smit, M Tang, G Roncador, L Maestre, E Conde, F Lopez-Rios, H C Clevers, M Sanchez-Cespedes
Oncogene 2006 Sep;26(11):1616-1625
Oncogene 2006 Sep;26(11):1616-1625
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 49
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in cells in seminiferus ducts.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
