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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB4325-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fc gamma RIII (CD16) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Fc gamma RIIIA/CD16a in direct ELISAs. In direct ELISAs, 60% cross-reactivity with Fc gamma RIIIB/CD16b is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P08637- Isotype
- IgG
- Antibody clone number
- 1001049
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Fc gamma RIIIA/CD16a by Western Blot. Western blot shows lysates of human lung tissue and human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Fc gamma RIIIA/CD16a Monoclonal Antibody (Catalog # MAB4325) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fc gamma RIIIA/CD16a at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Fc gamma RIIIA/CD16a in Human PBMCs. Fc gamma RIIIA/CD16a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Fc gamma RIIIA/CD16a Monoclonal Antibody (Catalog # MAB4325) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Fc gamma RIIIA/CD16a in Human Spleen. Fc gamma RIIIA/CD16a was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human Fc gamma RIIIA/CD16a Monoclonal Antibody (Catalog # MAB4325) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Fc gamma RIIIA/CD16a in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Fc gamma RIIIA/CD16a Monoclonal Antibody (Catalog # MAB4325) or (B) Mouse IgG2A isotype control antibody (Catalog # MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.
