12157-1-AP

antibody from Invitrogen Antibodies

Targeting: TCN2 D22S676, D22S750, TC2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12157-1-AP

Provider

Invitrogen Antibodies

Product name

TCN2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MRHLGAFLF LLGVLGALTE MCEIPEMDSH LVEKLGQHLL PWMDRLSLEH LNPSIYVGLR LSSLQAGTKE DLYLHSLKLG YQQCLLGSAF SEDDGDCQGK PSMGQLALYL LALRANWHDH KGHPHTSYYQ YGLGILALCL HQKRVHDSVV DKLLYAVEPF HQGHHSVDTA AMAGLAFTCL KRSNFNPGRR QRITMAIRTV REEILKAQTP EGHFGNVYST PLALQFLMTS PMRGAELGTA CLKARVALLA SLQDGAFQNA LMISQLLPVL NHKTYIDLIF PDCLAPRVML EPAAETIPQT QEIISVTLQV LSLLPPYRQS ISVLAGSTVE DVLKKAHELG GFTYETQASL SGPYLTSVMG KAAGEREFWQ LLRDPNTPLL QGIADYRPKD GETIELRLVS W (1-400 aa encoded by BC011239)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat liver tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).