H00006045-M03
antibody from Abnova Corporation
Targeting: RNF2
BAP-1, BAP1, DING, HIPI3, RING1B, RING2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00006045-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006045-M03, RRID:AB_1112104
- Product name
- RNF2 monoclonal antibody (M03), clone 3G6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant RNF2.
- Antigen sequence
PSNKRTKTSDDSGLELDNNNAAMAIDPVMDGASEI
ELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSK
YLAVRLALEELRSKGESNQMNLDTASEKQ- Isotype
- IgG
- Antibody clone number
- 3G6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in human stomach.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in Raw 264.7(Cat # L024V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RNF2 expression in transfected 293T cell line by RNF2 monoclonal antibody (M03), clone 3G6.Lane 1: RNF2 transfected lysate (Predicted MW: 36.52 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RNF2 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to RNF2 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol