PAB3288

antibody from Abnova Corporation

Targeting: NEK7

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3288

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3288, RRID:AB_1577656

Product name

NEK7 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of NEK7.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.

Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J
The Journal of biological chemistry 2003 Sep 12;278(37):34897-909

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Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3.

Kimura M, Okano Y
Cytogenetics and cell genetics 2001;94(1-2):33-8

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of NEK7 (arrow) using NEK7 polyclonal antibody (Cat # PAB3288).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK7 polyclonal antibody (Cat # PAB3288) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)