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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-47007 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK7 Monoclonal Antibody (HL1348)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Store as concentrated solution.
- Antibody clone number
- HL1348
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEK7 in Various whole cell extracts (30 µg) separated by 12% SDS-PAGE. Membrane was blotted with NEK7 Monoclonal antibody (Product # MA5-47007) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NEK7 in HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NEK7 stained by NEK7 monoclonal antibody (Product # MA5-47007) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NEK7 in paraffin-embedded mouse muscle. Samples were incubated in NEK7 Monoclonal antibody (Product # MA5-47007). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NEK7 in paraffin-embedded mouse heart. Samples were incubated in NEK7 Monoclonal antibody (Product # MA5-47007). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
