Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-87769 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIF14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LENGNNKAAS VQEEFMDAVC DGVGLGMKIL LDSGLEKAKE LQHELFRQCT KNEVTKEMKT NAMGLIRSLE NIFAESKIKS FRRQVQEENF EYQDFKRMVN RAPEFLKLKH CLEKAIEIII SALKGCHSDI NLLQTCVESI RNLASDFYSD FSVPSTSVGS YESRVTHIVH QELESLAKSL LFCFESEESP DLLKPWETYN QNTKEEHQQS KSSGIDGSKN KGVPKRVYEL HGSSPAVSSE ECTPSRIQWV; Positive Samples: HeLa, HL-60; Cellular Location: Cytoplasm, Midbody, Nucleus, cytoskeleton, spindle
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.46 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using KIF14 Polyclonal antibody (Product # PA5-87769) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 15s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KIF14 in extracts of various cell lines using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KIF14 was performed in L929 cells using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KIF14 was performed in U-2 OS cells using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIF14 in paraffin-embedded human colon carcinoma using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIF14 in paraffin-embedded human appendix using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIF14 in paraffin-embedded human breast cancer using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIF14 in paraffin-embedded human stomach using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIF14 in paraffin-embedded rat ovary using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIF14 in paraffin-embedded rat heart using KIF14 Polyclonal Antibody (Product # PA5-87769) at a dilution of 1:100.