NB300-549

antibody from Novus Biologicals

Targeting: ADORA1 RDC7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-549

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-549, RRID:AB_10002337

Product name

Rabbit Polyclonal Adenosine A1R Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects Adenosine Receptor A1. This does not detect other AR subtypes.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 uL

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Inhibition of LPS-stimulated ecto-adenosine deaminase attenuates endothelial cell activation.

Kutryb-Zajac B, Mierzejewska P, Sucajtys-Szulc E, Bulinska A, Zabielska MA, Jablonska P, Serocki M, Koszalka P, Milczarek R, Jasztal A, Bartoszewski R, Chlopicki S, Slominska EM, Smolenski RT
Journal of molecular and cellular cardiology 2019 Mar;128:62-76

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Adenosine A(1) Receptor mRNA Expression by Neurons and Glia in the Auditory Forebrain.

Hackett TA
Anatomical record (Hoboken, N.J. : 2007) 2018 Nov;301(11):1882-1905

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CD73 is associated with poor prognosis in HNSCC.

Ren ZH, Lin CZ, Cao W, Yang R, Lu W, Liu ZQ, Chen YM, Yang X, Tian Z, Wang LZ, Li J, Wang X, Chen WT, Ji T, Zhang CP
Oncotarget 2016 Sep 20;7(38):61690-61702

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Effect of adenosine A1 receptor agonist on the enhanced excitability of spinal dorsal horn neurons after peripheral nerve injury.

Yamaguchi D, Terayama R, Omura S, Tsuchiya H, Sato T, Ichikawa H, Sugimoto T
The International journal of neuroscience 2014 Mar;124(3):213-22

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Adenosine receptor expression and function in bladder uroepithelium.

Yu W, Zacharia LC, Jackson EK, Apodaca G
American journal of physiology. Cell physiology 2006 Aug;291(2):C254-65

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Adenosine A(1) receptor-mediated presynaptic inhibition at the calyx of Held of immature rats.

Kimura M, Saitoh N, Takahashi T
The Journal of physiology 2003 Dec 1;553(Pt 2):415-26

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NB300-549] - Analysis showing positive staining in the cytoplasm of Rat brain tissue (right) compared with a negative control in the absence of primary antibody (left).

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (PA1041A, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.