H00029937-M04

antibody from Abnova Corporation

Targeting: NENF CIR2, SCIRP10, SPUF

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00029937-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00029937-M04, RRID:AB_10616201

Product name

NENF monoclonal antibody (M04), clone 7D10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant NENF.

Antigen sequence

MVGPAPRRRLRPLAALALVLALAPGLPTARAGQTP
RPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKG
VVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSL
DPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIV
GYTARRILNEDGSPNLDFKPEDQPHFDIKDEF


Isotype

IgG

Antibody clone number

7D10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NENF expression in transfected 293T cell line by NENF monoclonal antibody (M04), clone 7D10.Lane 1: NENF transfected lysate (Predicted MW: 18.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NENF is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol