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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
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Validation data
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- Product number
- 60131-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neudesin/NENF Monoclonal Antibody (4G9E12)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: QTPRPAERG PPVRLFTEEE LARYGGEEED QPIYLAVKGV VFDVTSGKEF YGRGAPYNAL TGKDSTRGVA KMSLDPADLT HDTTGLTAKE LEALDEVFTK VYKAKYPIVG YTARRILNED GSPNLDFKPE DQPHFDIKDE F (33-172 aa encoded by BC008823)
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4G9E12
- Vial size
- 150 µL
- Concentration
- 1.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 60131-1-IG (NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 60131-1-Ig (NENF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 60131-1-IG (NENF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cerebellum using 60131-1-IG (NENF antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cerebellum using 60131-1-IG (NENF antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 60131-1-Ig (NENF antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 60131-1-Ig (NENF antibody) at dilution of 1:50 (under 40x lens).
